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Attachment Factor™ (4Z0-201/4Z0-210)

Attachment Factor™ (4Z0-201/4Z0-210)

$ 62.00

$ 0.00

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Volume:
  • 10mL4Z0201
  • 100mL4Z0210
Unavailable
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Product Details

Attachment Factor™ promotes cell attachment to tissue culture surfaces

 

Attachment Factor™ (4Z0-210) is an extracellular matrix (ECM) product that promotes cell attachment to tissue culture surfaces and encourages correct polarity and cytoskeletal organization. Use of Attachment Factor™ is critically important when cultures are to be initiated, grown, passaged, or used within the Cell Systems Media family. Cell Systems media and reagents are Sterile, made with WFI and all components are cGMP and ISO Compliant.

The biologically active elements of Attachment Factor™ are derived by a chromatographic process from heat-inactivated charcoal-stripped fetal bovine serum, and delivered at 1X in a PBS-based HEPES-buffered gelatin vehicle. Cell Systems exclusively uses and strongly recommends the use of serum products 100% traceable to cattle processed in the US at USDA inspected facilities.

Purpose

Use of Attachment Factor™ improves plating efficiency, shortens "lag time" after passage, enhances growth rate, and promotes the organization of polarity and cytoskeletal architecture, and can dramatically increase cell yield during cell culture expansion. Use with all cultures except Melanocyte and Keratinocyte cells.

Instructions for Use:

See the . 

Handling and Storage

Store Attachment Factor™ at <-20°C. Once product is opened, shelf life is 30 days at refrigerated temperatures (2-8°C). Warm to 37°C in water bath before each use. Refrigerate immediately after use.

For use of Attachment Factor™ in passaging and plating of cells, please see our  

Companion Products


Passage Reagent Group™


Bac-Off®


Cell Freezing Medium


Citations

  • Kim et al. Intl Journal of Biological Macromolecules, 2018

  • Wang et al. IOVS, 2018

  •  Wang & Law, Cellular Immunology, 2018

  • Saif, (Dissertation) 2016
  • Avril et al. mBio, 2016

  •   Lee et al. Toxicology Research, 2016

  • "  Herland et al, PLoS One, 2016

  •   Paradis et al. MethodsX, 2015

  •   Kim and Bynoe, Molecular Neurobiology, 2014

  • ""  Safi et al. BMC Medical Genomics, 2014

  • Gerlach et al. Peptide Science, 2013

  • Zhang et al. Molecular Vision, 2013

  •   Kodela et al. Medchemcomm, 2013

  • Maeng et al. PLoS One, 2013

  • Gonzalez-Velasquez et al. J Adhesion Science & Technology, 2012

  •  Kusuhara et al. PLoS One, 2012

  • ""  Bhattacharjee et al. PLoS One, 2012

  • Sullivan et al. Molecular Pharmacology, 2012

  • Mitchell et al. J Neurochemistry, 2011

  • Rush et al. Experimental Cell Research, 2007

  • Nestoridi et al. J Thrombosis and Hemostasis, 2005

  • Steinle et al. J Biological Chemistry, 2003

  • Steinle and Granger, Autonomic Neuroscience, 2003

  • " Lee et al. J Biological Chemistry, 2003

  • Lee et al. J Biological Chemistry, 2002

  • "" Jiminez et al. British Journal of Haematology, 2001

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